Molecular pathology test protocol

The Neuropathology department carries out around 400 molecular pathology tests a year. These include DNA testing of common brain tumour prognostic markers such as MGMT promoter methylation status and the sequencing of IDH1, IDH2 and BRAF V600E using state of the art pyrosequencing. 1p/19q co-deletion and c-MYC proliferation tests are performed using fluorescence in situ hybridisation (FISH). The number of markers tested is rapidly increasing. Please contact the department for a current list.

Please follow this protocol for sending samples for Molecular Pathology tests to King’s College Hospital Department of Clinical Neuropathology.

1. Phone us in advance

Contact the Neuropathology Laboratory at King’s College Hospital on 020 3299 1957 to let staff know when to expect delivery. If there is no answer, then phone the department office on 020 3299 1955 and leave a message. The department is open from 8.30am to 5pm, Monday to Friday.

2. Sample Requirements and Packaging

Ten unstained formalin fixed paraffin embedded (FFPE) tissue sections, cut at 4-5µm thick (from a representative block of tumour) are required for all molecular pathology tests. If you have insufficient material or if you require multiple tests on the same samples please call the laboratory on 020 3299 1957 to discuss any additional requirements prior to sending.

3. How to package and transport the sample

The unstained slides must be packed into clean slide carriers taped shut to ensure they are not damaged during transit. A letter explaining the clinical details should accompany the sample.

Post in a padded envelope to:
Department of Clinical Neuropathology, 1st Floor, Academic Neuroscience Centre,
King’s College Hospital,
Denmark Hill,
London SE5 9RS

Contact us

If you have any difficulties or questions, in the first instance contact Ross Laxton on 020 3299 1957.

Alternatively, phone Lawrence Doey, Laboratory Manager, on 020 3299 1951.