Muscle and nerve biopsy protocol

The department receives around 250 muscle and around 30 nerve biopsies each year, and serves as the main referral centre for muscle and nerve diseases. We use comprehensive immuno-stains for inflammatory myopathy and sarcolemmal protein for muscular dystrophy. A panel of immunohistochemistry, semi-thin sections and electron microscopy is used to investigate the nerve biopsies. There are regular weekly meetings with neurologists and neurophysiologists to discuss cases.

Please follow the protocol for performing and sending muscle and nerve biopsies to King’s College Hospital Department of Clinical Neuropathology.

1. Phone us in advance

Contact the Neuropathology Laboratory at King’s College Hospital on 020 3299 1957 the day before the procedure and give a rough time when the biopsy is being taken and the patient’s name. If there is no answer, then phone the department office on 020 3299 1955 and leave a message. The department is open from 8.30am to 5pm, Monday to Friday.

Please phone the department as soon as possible to notify us of any changes or cancellations.

2. How to take biopsies

Unless specifically indicated/contra-indicated by the patient’s condition, the muscle of choice to biopsy is the quadriceps or biceps and the nerve of choice is the sural. The volume of tissue required is approximately 1 cubic cm for muscle and 1-3cm in length for nerve. The muscle should be removed as an open biopsy without local use of diathermy or cortory. The sample must not be clamped or tied to a stick. Biopsies obtained by the use of ‘guns’ should be avoided since the reconstruction necessary makes sample interpretation very difficult. The nerve should be removed by open biopsy.

Two biopsies can be sent at the same time, as long as they reach the laboratory within the required timeframe. It will be cheaper to send two together, rather than using a separate courier for each sample.

3. When to take biopsies

The biopsies should all to be done in the morning, and preferably as early as possible, in order to allow a reasonable time for the dissection and selection of samples for freezing and other procedures. We do not accept biopsies arriving later than 4.30pm at our laboratory.

4. How to package and transport the sample

The sample should be placed in a universal container without anything else added. A letter explaining the clinical details, plus any other useful information such as the EMG report etc, must accompany the sample. It should be sent by courier to arrive in this department within one hour of taking the biopsy. The packaging must consist of:

  • A primary receptacle
  • A leak-proof secondary packaging
  • An outer packaging with minimum dimensions of 100x100mm
  • The complete package shall be capable of withstanding a drop of 1200mm.

The package must be labelled UN3373, contained within a diamond shape with minimum dimensions of 50x50mm. The proper shipping name – Biological Substances, Category B – must be marked adjacent to the label.

It is the sender’s responsibility to comply with these regulations.

Mark the package as:
‘Urgent sample from {insert department and hospital} for Department of Clinical Neuropathology, 1st Floor, Academic Neuroscience Centre, King’s College Hospital, Denmark Hill, London SE5 9RS. Telephone: 020 3299 1957’

Tell the courier that the Academic Neuroscience Centre is located on Denmark Hill. There is a buzzer entry system and they will need to press the button marked ‘Clinical Neuropathology’. The secretary will then direct them to the laboratory on the first floor. Please phone the laboratory on 020 3299 1957 when the courier has left your hospital. If the courier has difficulty finding the department, or nobody answers the buzzer, they should phone the laboratory for local directions or so someone from the laboratory can meet them at the entrance to the building.

Contact us

If you have any difficulties or questions, in the first instance contact Stefan Buk or Philip Green on 020 3299 1957.

Alternatively, phone Lawrence Doey, Laboratory Manager, on 020 3299 1951.